Fouchets test – bile pigments(urobilinogen and porphobilinogen) Hay’s Sulphur test- Bile salts Benedicts test – Blood sugar Rothera’s test – Ketone bodies Biuret test – Protein Biuret reagent- CuSO4,Sodium potassium tartarate,Potassium iodide in sodium hydroxide Test for glucose Benedict’s test …
Category Archives: Biochemistry
Biochemical tests
Posted by admin on June 23, 2011 No comments
Glycogen storage disorders mnemonic
Posted by admin on February 25, 2011 No comments
Metallo-enzymes
Posted by admin on February 23, 2011 No comments
Metals and corresponding enzymes is one of the frequently asked questions in MCQ exams. Zinc Carbonic anhydrase Dehydrogenases(LDH,Alcohol dehydrogenase) Kinases Peptidases Magnesium Hexokinase Enolase Copper Tyrosinase Cytochrome Oxidase Iron Cytochrome oxidase Catalase Peroxidase Molybdenum Xanthine oxidase Manganese Superoxide dismutase Calcium …
Co Factors of enzymes
Posted by admin on November 15, 2010 No comments
Rate Limiting steps in biochemical reactions.
Posted by admin on November 15, 2010 No comments
Glucose transporters
Posted by admin on October 29, 2010 No comments
Glucose enters cells by facilitated diffusion or by secondary active transport with Sodium(in intestine and kidneys) Glucose transporters(Glut 1-7) are responsible for facilitated diffusion of glucose across cell membranes. They differ from sodium dependant glucose transporters(SGLT1 and 2) Glucose Transporters …
Glycogen Storage diseases
Posted by admin on October 15, 2010 1 comment
Liver Glycogenoses Disorders with hepatomegaly and hypoglycemia Type I-Von Gierke -Glucose 6 phosphatase Type III-Cori/Forbe limit dextrinosis- Debranching enzyme deficiency Type VI-Her’s Liver phosphorylase Type IX-Phosphorylase Kinase deficiency Type XI-Fancon/Bickel Glut 2 deficiency With Liver cirrhosis Type IV-Anderson’s -Branching enzyme …
Classification of Hormones
Posted by admin on September 30, 2010 No comments
Lipoproteins
Posted by admin on September 26, 2010 No comments
Cholesterol,Triglycerides,Phospholipids. Chylomicron’s Deliver dietery TGL to peripheral tissues. Deliver cholesterol to liver in form of chylomicron remnant’s Secreted by intestine Excess causes pancreatitis,lipemia retinalis,eruptive xanthomas. VLDL Deliver’s hepatic TGL to peripheral tissues. Secreted by liver. Excess causes pancreatitis. LDL Deliver’s …
Human Porphyrias
Posted by admin on September 26, 2010 No comments
Inherited or acquired disorders of heme biosynthetic pathway Acute(hepatic porphyrias)- acute neurological attacks-seizures,psychosis,backpain,abdominal pain,acute polyneuropathy) Erythropoietic porphyrias- Skin problems,lightsensitive blistering rash and increased hair growth. Variegate porphyria-Both neurological and skin features. Porphyria’s -Disease of 5P’s Pain abdomen,Psychological disturbances,Polyneuropathy,Purple urine,Precipitated by …
Mucopolysaccharidoses
Posted by admin on September 26, 2010 No comments
Type I-Hurler’s Autosomal recessive. Alpha L Iduronidase deficiency. Coarse face,Thick skin,severe MR. Gargolylism,Corneal opacity. Dermatan and Heparan sulfate in urine. Type -II Hunter’s Xlinked recessive. Iduronate sulfatase deficiency. Mild Hurler’s+aggressive behavior,deafness. No corneal opacity. Mnemonic:Hunter aims for X and has …
Lipid storage diseases
Posted by admin on September 26, 2010 No comments
They are lysosomal storage diseases Also called sphingolipidoses Rate of synthesis is normal only degradation is affected. Usually death occurs soon after birth. Except Fabrys disease(XLR) and Metachromatic leukodystrophy(No inheritence pattern) all of them are AR Fabry’s disease Alpha Galactosidase …
Water soluble vitamins
Posted by admin on September 17, 2010 No comments
Vitamin B complex and Vitamin C are the only fat soluble vitamins. Vitamin B complex Vitamin B1-Thiamine(beriberi) Thiamine is pyruvate dehydrogenase cofactor,Erythrocyte transketolase levels to test deficiency. Vitamin B2-Riboflavin Vitamin B3-Niacin(Pellagra) Niacin is formed from tryptophan,deficient in maize eaters. Vit …
Fat soluble Vitamins
Posted by admin on September 16, 2010 No comments
Disorders of amino acid metabolism
Posted by admin on September 16, 2010 No comments
Alkaptonuria(Ochronosis) Congenital deficiency of Homogentisic acid oxidase, needed for degradation of tyrosine.Alkaptone bodies formed cause urine to turn black on standing,connective tissue also becomes black(ochronosis) Phenyl Ketonuria(PKU) Phenylalanine————>tyrosine———–>melanin Autosomal recessive disease.Phenyl alanine hydroxylase deficiency,phenylalanine accumulates, tyrosine becomes an essential Amino …
