Category Archives: Microbiology

The structure of a virus

A virus is an infectious particle which can replicate only inside a cell using the synthetic mechanism of the cell. A virus when outside a living cell is usually dormant, and shows no signs of life.Almost two centuries had passed …

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Types of human herpes viruses and associated diseases.

Human herpes viruses are 8 in number, they are associated with a number of diseases like fifth diseases, sixth disease,herpes zoster etc.

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Bacterial diarrhea: Invasive vs Non Invasive diarrhea.

Bacterial diarrhea’s can be classified into invasive and non invasive, this classification has an important role in determining treatment modalities. Non Invasive Diarrhea Caused by release of toxins from bacteria resulting in disruption of secretory process. Characterized by watery diarrhea. …

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Important parasites

Parasites causing malabsorption in adults Giardia Lamblia E.Histolytica H.Nana Strongyloides Cyclospora Malabsorption in children G.Lamblia Isospora belli Cryptosporidium Ancylostoma duodenaLE E.Histolytica Capillaria phillipinensis Man is the defenitive host in most parasitic infections except in: Echinococcus granulosus Plasmodium Taenia Solium(man is …

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Dimorphic fungi

Can grow either as yeast or as mould depending on env conditions and temperature Usually yeast at body temperature(37C) and as filamentous forms at room temperature(27C) Blastomycetes Paracoccidiomyces Blastomyces Sporothrix Candida albicans(not other candida) Histoplasma capsulatum Penicillin marneffi

Nematodes,Cestodes and trematodes

Nematodes Round worms Enterobius Vermicularis-Pin worm Ascaris Lumbricoids-Giant round worm Trichinella spiralis Trichiuris trichiura Dracunculus mediensis Onchocerca volvulus Loa loa Wuchereria bancrofti Toxocara canis Hook worms Strongyloides stercoralis Ancylostoma duodenale Necator americanus Routes of infection Ingested:Enterobius,Ascaris,Trichinella,Trichiuris Cutaneous:Strongyloids,Ancylostoma,Necator(Hookworms) Cestodes/Tapeworms Tinea solium …

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Hepatitis Viruses

Hepatitis A(Infectious hepatitis) RNA picorna virus Feco-oral route of infection Mild self limiting disease,active immunization available. Hepatitis B(Serum hepatitis) DNA hepadnavirus Parenteral/sexual transmission 1-2% mortality,95% cases resolve Primary HCC,cirrhosis Hepatitis C(Post transfusion hepatitis) RNA flavi virus Parenteral/Sexual transmission a/c disease …

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Corynebacterium diptheriae

Gram positive rods,arranged in palisades V or L shaped formations,Cuneiform(chinese letter)pattern. Metachromatic granules(volutin,Babes ernst,polar granules) stained by -Alberts,Neisser’s,Ponder’s stains. Form black colonies on tellurite agar(in 2 days),grow rapidly in Loeffler’s serum slope Virulence factors-Diptheria toxin inhibits protein synthesis in cell. …

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Mycobacteria

Mycobacterium tuberculosis Gram positive rod,acidfast,stains red on zeihl Neilson staining due to high mycolic acid. Obligate aerobe,LJ medium for growth Produces Niacin Virulence factor:Cord factor allows growth in extended chains. Causes Pulmonary TB,primary disease affects lung bases, reactivation disease occur …

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Clostridium Properties

Clostridia are one of those bacilli which are gram positive. Obligate anaerobes,spore forming and highly pleomorphic. Shows stately motility and have peritrichate flagella. Cl. Welchi and Cl.tetani type VI do not have peritrichate flagella. Clostridia are normal residents of GIT …

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Ecoli Properties

Gram negative rod,Facultative anaerobic,motile,Oxidase negative Antigens:Somatic antigen O,capsular antigen K,Flagellar antigen H. Types Divided into 5 types. 1.Enteropathogenic Ecoli(EPEC) Non Invasive Adheres tightly to enterocytes using intimin leading to loss of microvilli. Causes infantile diarrhoea. 2.Enterotoxigenic Ecoli(ETEC) Noninvasive Most important …

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Malaria features

P.falciparum Malignant Tertian fever-occurs in 48 hour intervals Reticulocytes,normal and mature RBC’s are affected Banana shaped gametocyte,highest parasite burden,Mature ring trophozoites Maurers dots. Malignant tertian,cerebral malaria,blackwater fever(DIC and renal failure),Algid malaria(circulatory failure) P.Vivax Benign Tertian malaria Infects reticulocytes Schuffner’s dots,mature …

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Colony appearances of bacteria

Bacillus anthracis Bamboo stick medusa head Inverted fir tree appearance Tip:Anthrax usually related to cow,imagine a cow tied to a fir tree/Bamboo. Bordetella Pertussis Thumb print Besected pearls Mercury drops Aluminium paint These are the typical appearances of pertussis Tip:Cough …

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Biological Stains

Congo Red Amyloid Apple green birefringence in polarised light because of beta pleated sheets Giemsa Borrelia Plasmodium Trypanosomes Chlamydia. PAS Glycogen Mucopolysaccharides Used to diagnose whipples disease Zeihn Neilsen Acid fast baceria India Ink Cryptococcus neoformans Silver stain Fungi Legionella

Bacteria features

Organisms which do not gram stain well These Rouges May Microscopically Lack Color. Treponema,Rickettsia,Mycobacteria,Mycoplasma,Legionella pneumophila,Chlamydia. Oh My no cellwall. Intracellular Organisms Obligate Intracellular Mycoplasma,Rickettsiae. RiCh organisms stay inside Facultative Intracellular Sally Myc and Bruce Neednot Live FreeLY. Salmonella,Mycobacterium,Brucella,Neisseria,Listeria,Franciscella,Legionella,Yersinia. Obligate anaerobes …

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Mycoplasma and Chlamydia

Often confused topic. Mycoplasma. Smallest free living and self replicatingĀ  organism on lab media. Lack rigid cellwall, so highly pleomorphic. Bounded by triple layered membrane containing sterol, so they require sterol for growth. Easily pass through bacterial filters. Completely resistant …

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Named diseases

Piroplasmosis/texas fever-Babesia Katayama disease-Schistosoma Japonicum Omsk fever-flaviviridae Gaol fever-rick prowazaki Pink eye-H.egipticus Bagdad sore/delhi boil/oriental sore-leishmania tropica carrions disease/peruvian wart-bartonella bacilliformis hebra nose-K.Rhinosleromatis milkers node-paravaccinia vagabonds disease-pediculosis corporis buruli ulcer-Myc.Ulcerans Coconut cake rectum-Trichiuris trichura Pseudohemoptysis-serratia walking pneumonia-myc.pneumonia pontiac fever-legionella weils …

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Bacteria and their alternate names

Image via Wikipedia Making absurd and vivid associations is the key to memory. Bordetella- Bordet Gengou bacillus Clostridium tetani-Nicolaire’sĀ  bacillus(Nicolas cage, nail stick injury on his foot) Corynebacterium diptheriae-Kelbs Loeffler’s bacillus(Diptheria near tonsil>tonsil is like a club>Kleb) Coryn pseudotuberculosis-Priesz Nocard …

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