Methods of measuring body fluid volumes

60% of TBW is water- 20% ECF and 40%ICF

Out of 20% ECF- 15% is Interstitial fluid and 5% is plasma.

Total blood volume is 8% including plasma +cells.

Method’s to measure.

Plasma volume

  • Evans blue-T1824
  • Serum albumin labelled with radioactive iodine.


  • Inulin
  • Mannitol
  • Sucrose


  • Deuterium oxide
  • Tritium oxide
  • Aminopyrine

Red cell volume

Tagged RBC’S

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